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stable cell line generation human embryonic kidney hek293  (ATCC)


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    ATCC stable cell line generation human embryonic kidney hek293
    Stable Cell Line Generation Human Embryonic Kidney Hek293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 22011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+cell+line+hek293/pm41807755-174-3-11?v=ATCC
    Average 99 stars, based on 22011 article reviews
    stable cell line generation human embryonic kidney hek293 - by Bioz Stars, 2026-07
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    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in <t>HEK293</t> cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.
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    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in <t>HEK293</t> cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.
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    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in <t>HEK293</t> cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.
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    cell lines human embryonic kidney hek293s gnti cell line atcc crl  (ATCC)
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    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in <t>HEK293</t> cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.
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    Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in HEK293 cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.

    Journal: International Journal of Biological Sciences

    Article Title: Targeting QRICH1 suppresses epithelial-mesenchymal transition and tumor growth in liver cancer through the Connexin43/USP1 mediated Snail1 stabilization

    doi: 10.7150/ijbs.133080

    Figure Lengend Snippet: Mechanistic association between QRICH1 and USP1 or Snail1 in Huh7 and SK-Hep-1 cells through their binding. (A) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. ***p<0.001 vs untreated control. (B) Effect of QRICH1 depletion on USP1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (C) Interaction between QRICH1 and USP1 in HEK293 cells by Flag IP and IB. (D) The direct binding between QRICH1 and USP1 in Huh7 and SK-Hep-1 cells by IP. (E) Effect of USP1 depletion on QRICH1 and Snail1 expression in Huh7 and SK-Hep-1 cells by qRT-PCR. **p<0.01, ***p<0.001 vs untreated control. (F) Effect of USP1 depletion on QRICH1 expression in Huh7 and SK-Hep-1 cells by Western blotting. (G) Effect of QRICH1 depletion and USP1 overexpression on USP1 and Snail1 in Huh7 and SK-Hep-1 cells. (H) Effect of USP1 depletion on the binding to QRICH1 and Sanil1 in Huh7 and SK-Hep-1 cells.

    Article Snippet: The liver cancer cell lines Huh7, SK-Hep-1, HepG2, SNU-449 and human embryonic kidney cell line HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea).

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Control, Western Blot, Over Expression

    USP1 dependent regulation of Snail1 ubiquitination and specification of ubiquitin chain linkages. (A) Detection of Snail1 ubiquitination in Huh7 and SK-Hep-1 cells transfected with siQRICH1 and treated with MG132. (B) Assessment of ubiquitinated Snail1 in SNU-449 and HEK293 cells coexpressing the indicated vectors following a 4 h MG132 treatment, using Flag IP and subsequent IB analysis. (C) Determination of Snail1 ubiquitin-chain linkage patterns in HEK293 cells cotransfected with Flag-Snail1, Myc-USP1, and the designated His-Ub variants (WT, K48-only or K63-only). (D) Validation of ubiquitin-linkage in HEK293 cells expressing Flag-Snail1, Myc-USP1, and the indicated His-Ub plasmid (K6, K11, K27, K29, K33).

    Journal: International Journal of Biological Sciences

    Article Title: Targeting QRICH1 suppresses epithelial-mesenchymal transition and tumor growth in liver cancer through the Connexin43/USP1 mediated Snail1 stabilization

    doi: 10.7150/ijbs.133080

    Figure Lengend Snippet: USP1 dependent regulation of Snail1 ubiquitination and specification of ubiquitin chain linkages. (A) Detection of Snail1 ubiquitination in Huh7 and SK-Hep-1 cells transfected with siQRICH1 and treated with MG132. (B) Assessment of ubiquitinated Snail1 in SNU-449 and HEK293 cells coexpressing the indicated vectors following a 4 h MG132 treatment, using Flag IP and subsequent IB analysis. (C) Determination of Snail1 ubiquitin-chain linkage patterns in HEK293 cells cotransfected with Flag-Snail1, Myc-USP1, and the designated His-Ub variants (WT, K48-only or K63-only). (D) Validation of ubiquitin-linkage in HEK293 cells expressing Flag-Snail1, Myc-USP1, and the indicated His-Ub plasmid (K6, K11, K27, K29, K33).

    Article Snippet: The liver cancer cell lines Huh7, SK-Hep-1, HepG2, SNU-449 and human embryonic kidney cell line HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea).

    Techniques: Ubiquitin Proteomics, Transfection, Biomarker Discovery, Expressing, Plasmid Preparation

    Effect of QRICH1 overexpression on proliferation, migration and EMT and cytoskeleton related proteins in SNU-449 and HEK293 cells (A) QRICH1, USP1, and Snail1 mRNA levels in SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. ***p < 0.001 vs. untreated control. (B) Clonogenic capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. **p<0.01, ***p<0.001 vs untreated control. (C) Migratory capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. Scale bar, 200 μm. *p<0.05 vs untreated control. (D) EMT- and cytoskeleton-related protein profiles of SNU-449 and HEK293 cells with QRICH1 overexpression. *p<0.05, **p<0.01, ***p<0.001 vs untreated control.

    Journal: International Journal of Biological Sciences

    Article Title: Targeting QRICH1 suppresses epithelial-mesenchymal transition and tumor growth in liver cancer through the Connexin43/USP1 mediated Snail1 stabilization

    doi: 10.7150/ijbs.133080

    Figure Lengend Snippet: Effect of QRICH1 overexpression on proliferation, migration and EMT and cytoskeleton related proteins in SNU-449 and HEK293 cells (A) QRICH1, USP1, and Snail1 mRNA levels in SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. ***p < 0.001 vs. untreated control. (B) Clonogenic capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. **p<0.01, ***p<0.001 vs untreated control. (C) Migratory capacity of SNU-449 and HEK293 cells transfected with QRICH1 overexpression plasmid. Scale bar, 200 μm. *p<0.05 vs untreated control. (D) EMT- and cytoskeleton-related protein profiles of SNU-449 and HEK293 cells with QRICH1 overexpression. *p<0.05, **p<0.01, ***p<0.001 vs untreated control.

    Article Snippet: The liver cancer cell lines Huh7, SK-Hep-1, HepG2, SNU-449 and human embryonic kidney cell line HEK293 were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea).

    Techniques: Over Expression, Migration, Transfection, Plasmid Preparation, Control